The Caldwell Fire of July 2020 burned 69% of Lava Beds National Monument, which is home to 16 bat species across its volcanic landscape. While fire events have been shown to benefit bats in densely forested areas due to increased openness of foraging habitat, the effects of fire on bats in the shrub and grassland communities prevalent at Lava Beds are largely unknown. We sought to determine the Caldwell Fire’s effects on bat acoustic activity and identify which species, if any, may face increased vulnerability in a post-fire landscape. We collected bat acoustic data in August and September 2020 across 7 sites which were geographically and temporally matched to pre-fire surveys, then compared activity at the 3 burned and 4 unburned sites. The response to the Caldwell Fire varied by species, with some showing increased activity while others decreased or remained stable. These results help inform landscape-scale conservation needs, though additional work is required to identify long-term fire impacts to bats in a xeric environment, particularly since an additional 27% of Lava Beds National Monument burned in the Antelope Fire of 2021.