Snake Fungal Disease (SFD, ophidiomycosis), caused by the fungus Ophidiomyces ophidiicola, has emerged as a threat to the health of snake populations worldwide. For the first time in 2019, the California Department of Fish and Wildlife (CDFW) confirmed the presence of clinically significant SFD in a free-ranging California kingsnake (Lampropeltis californiae) from Amador County admitted for wildlife rehabilitation and in an invasive Florida banded watersnake (Nerodia fasciata pictiventris) found during targeted removal efforts in Sacramento County. These detections demonstrated the need for additional surveillance to characterize the distribution and prevalence of SFD and O. ophidiicola across snake populations in California. From July 2021 through June 2022, we surveyed free-ranging snake populations and snakes submitted for wildlife rehabilitation from 25 counties and collected swab samples from 243 captured snakes to test for the presence of O. ophidiicola DNA using qPCR. To date we have confirmed the presence of O. ophidiicola DNA from another free-ranging California kingsnake admitted for wildlife rehabilitation in Marin County in April 2022. Our results indicate that O. ophidiicola is present and infecting free-ranging snakes in disjunct areas of California, affirming the need to establish and maintain long-term systems for surveillance, education, and disease prevention.