There are few pieces of land left in the west that remain unknown. Fewer still that exist entirely in private ownership, span 85 square miles across four counties, and that are located less than 50 miles from San Francisco. The magnitude of time and labor required to canvas 50,000 acres of wilderness using traditional survey methods can be daunting. Using relatively simple GIS tools, landscape modeling, eDNA metabarcoding, and finally traditional survey methods as a validation tool, WRA was able to quickly and economically build a landscape level picture of targeted species distribution across a vast and rugged property. This talk will focus on the approach, methods, and findings of one of the most extensive validated eDNA case studies that occurred at what stands to be a conservation stronghold in an increasingly urbanized region.