Climate change, fire suppression, and human-caused ignition have led to an increasing trend in large wildfire frequency and area burned in the western United States. With these changes in wildfire trends there is a need to study the long term effects of wildfire, especially their impact on freshwater aquatic ecosystems. Wildfire is known to release metals from the environment which poses negative ecological and toxicological implications for aquatic organisms when they are washed into streams. Metals from this runoff may accumulate in the tissues of aquatic organisms where they may persist in the food web. This study will use historical samples collected from three streams before and after the 2021 Dixie fire in Northern California to investigate the long-term effects of wildfire on aquatic invertebrates. Contamination of freshwater systems poses serious ecotoxicological health hazards to aquatic biota and stream integrity as metals become bioavailable and bioaccumulate. This study will provide insight into potential metal accumulation and persistence in aquatic invertebrates following wildfire, and how these organisms could serve as bioindicators for restoration and mitigation efforts in fire-disrupted freshwater ecosystems. Metal analysis has been conducted using inductively-coupled plasma mass spectrometry and I will share some preliminary data.