An essential operational goal for the California High-Speed Rail (HSR) project is the use of zero emission trains operating on 100% renewable energy, between San Francisco to Los Angeles within 3-hours. The HSR Authority recognizes that unintended impacts associated with project must be addressed in parallel with construction and operational goals. Wildlife corridors and safe crossings have been the focus of the early planning, design and permitting decisions. This presentation will highlight mitigation efforts to maintain habitat connectivity and will touch on the design of wildlife underpass corridors, bridges, and viaducts to create a permeable rail line. The placement of 338 various dedicated wildlife crossing structures aims to avoid habitat fragmentation. Construction is nearing completion along the first 119-mile segment in the Central Valley, which covers 15 different habitat types. A Wildlife Crossing Monitoring Program has been developed to better understand how the SJKF and other terrestrial wildlife species interact with the designs of each crossing structure. The findings will be made available to CDFW, USFWS, Caltrans and other agencies to improve other wildlife connectivity programs and guidance documents.