Aqueducts can constrain some ground-based wildlife species’ movement patterns and gene flow for certain suites of species. In 2020 the California Department of Water Resources launched an effort to prepare two Habitat Conservation Plans (HCPs) to cover Operations and Maintenance (O&M) Activities for the State Water Project (SWP), which uses aqueducts to supply water from Northern California to Southern California. To identify the suite of affected species and extent of potential constraints, and to evaluate potential hot spots and opportunities, a wildlife movement study and analysis was performed across 294-linear miles of the aqueduct. The analysis considered focal and other key species, species habitat modeling, priority area mapping for connectivity, crossing type usage analysis, and identification of “important areas” for wildlife movement. Based on the results of the analysis, the study provided a discussion of the effects of structures and other barriers and issues and offered solutions and recommendations for improving wildlife movement. These were used to identify species-specific and cumulative hot spots to focus mitigations at. These include installation of additional crossing structures and other wildlife movement corridor improvements to support and enhance wildlife movement and genetic flow across the aqueduct system and are described in this Poster.