Many populations of generalist species are composed of specialist individuals that vary in resource use. In urban environments, niche variation may be widespread due to landscape heterogeneity, novel food resources, high species densities, and intraspecific trait variation. Although theory provides a rationale for the existence of strong individual diet specialization in urban populations, few studies have considered among-individual differences in urban animal diets. We compared niche breadth and dietary specialization in an urban and nonurban population of coyotes (Canis latrans) using stable isotope analysis. We found that urban individuals had dietary niches nearly three times narrower than nonurban individuals and that the urban population showed greater among-individual variation in isotope values. Specifically, within-individual differences explained only 18% of total δ13C variation in urban coyotes versus 58% in nonurban coyotes, and 34% versus 44% of δ15N variation, indicating much stronger individual specialization in the urban population. However, coyotes sharing the same neighborhoods had relatively similar diets. Recognition of individual variation implies a small proportion of individuals could be disproportionately responsible for negative impacts on human interests. We conclude by discussing how identification of such 'problem individuals,' and their foraging strategies, can inform policies set by urban practitioners to mitigate human-coyote conflict.