Environmental DNA (eDNA) is a powerful, non-invasive tool for detecting wildlife, including rare and sensitive species. In 2025, River Partners performed eDNA sampling at 19 sites across more than 3000 acres in the San Joaquin Valley, capturing 1200 samples as part of our baseline monitoring effort to evaluate biodiversity on sites before and after restoration. eDNA offers key advantages, including the ability to detect cryptic or non-vocal species. However, it also presents limitations, such as a lack of genetic data for certain species and sensitivity to environmental conditions. One site of particular interest was the Tule Elk State Natural Reserve in California, where over 20 sensitive species have historically been detected. At this site, we conducted baseline monitoring using eDNA, wildlife camera traps, and acoustic recording units (ARUs). We collected soil-based eDNA samples from 12 transect locations, sampled twice per year, resulting in a total of 288 samples, and deployed 10 camera traps and 10 ARUs between April and July 2025. We will present data from each method and discuss the species that were detected, offering a more comprehensive understanding of the effectiveness of different monitoring techniques.

Anna holds a Master’s degree in Environmental Science and Management and brings over eight years of experience working with environmental non-profits to advance conservation and restoration efforts. Her background includes wildlife monitoring, project development, geospatial analysis, and science communication. She has led and contributed to research on various topics, including mountain lion habitat connectivity in California. Since joining River Partners in 2022, Anna has led the planning, implementation, and monitoring of riparian and upland restoration projects in California’s southern San Joaquin Valley. She specializes in designing site-specific, scalable restoration projects informed by scientific field trials.