Rangeland ecosystems support diverse wildlife yet remain difficult to monitor due to their vast scales, permitting constraints, and limited resources available to producers. This project investigates environmental DNA (eDNA) as a cost-effective, non-invasive tool for assessing wildlife biodiversity across Alameda and Contra Costa County rangelands. Over a three-year period, the Alameda County Resource Conservation District (ACRCD) and Contra Costa Resource Conservation District (CCRCD), in partnership with NRCS, are collecting eDNA samples from livestock ponds and creeks to detect vertebrate and invertebrate species presence and determine. Results are compared with traditional survey techniques (visual, trapping, camera) to evaluate efficiency, accuracy, and cost. The project aims to demonstrate that eDNA can complement or replace conventional wildlife surveys, support voluntary conservation by ranchers, and inform NRCS wildlife habitat management practices. By integrating eDNA into working lands conservation, this project seeks to expand producer participation, improve biodiversity data across East Bay rangelands, and create a foundation for using eDNA monitoring to evaluate habitat restoration and management success.