An essential operational goal for the California High-Speed Rail (HSR) project is the use of zero emission trains operating on 100% renewable energy, between San Francisco to Los Angeles within 3-hours. The HSR Authority recognizes that the unintended impacts associated with project must be addressed in parallel with construction and operational goals. Consequently, wildlife crossings have been incorporated into the HSR project design. This presentation highlights results of a pilot project to inform full implementation of the HSR Wildlife Crossing Monitoring Plan (Plan) designed to better understand how San Joaquin kit fox (Vulpes macrotis mutica) and other terrestrial wildlife species interact with 338 dedicated wildlife crossing structures installed within 15 different habitat types between Fresno and Bakersfield to avoid habitat fragmentation. We installed a total of 24 cameras: six cameras (three on each side of the crossing structure) at four locations. In order to assess the effectiveness of different camera models, and the number of cameras needed to meet the goals of the Plan, we tested four brands of candidate cameras and software programs. We include an assessment of AI-assisted wildlife photo processing with Megadetector and YOLOv8 GUI using the AddaxAI open-source application.